Diagnostic Enzymes

Clinical, biotech & research applications

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We uniquely offer precision fermentation, custom bioprocessing, and contract manufacturing solutions alongside our own range of diagnostic enzyme products used in clinical reagents and many other biotechnology and industrial applications.

These include Arylacylamidase, Beta-Hydroxybutyrate Dehydrogenase, and Salicylate Hydroxylase. These diagnostic enzymes are produced in bulk and used to produce Stanbio Chemistry-branded reagents and OEM reagents. They are also sold commercially to third-party clinical diagnostic companies for IVD production.

  • Arylacylamidase (A-010):Used for the enzymatic determination of acetaminophen in serum.
  • Beta-Hydroxybutyrate Dehydrogenase (H-010): Used for the enzymatic determination of ketone bodies in blood and serum.
  • Salicylate Hydroxylase (S-010): Used for the enzymatic determination of salicylate in serum.

Other enzymes successfully manufactured or in development at EKF Life Sciences include Uricase, Phenylalanine Dehydrogenase and Diaphorase.

EKF Life Sciences is home to a diverse community of biotechnology experts, scientists, and engineers. Our talented and dedicated team comprises individuals with deep-rooted knowledge, passion, and a collective commitment to advancing life sciences.

Their expertise spans a multitude of domains, from microbiology to molecular biology, ensuring a comprehensive and collaborative approach to every project. We welcome the active participation of our clients, as our experts not only drive discoveries but push the boundaries of what’s achievable.

The confluence of expertise and technology within our facilities amplifies our ability to undertake specialist development projects, paving the way for transformative breakthroughs.

 

Arylacylamidase (A-010)

Form

White, off-white, or yellow lyophilized powder.

Activity

≥ 15 U/mg powder.

Unit

One unit is defined as the amount of enzyme which catalyzes the conversion of 1 µmole of p-nitroacetanilide to p-nitroaniline at 30˚C, pH 8.5 under the conditions given in the assay procedure.

Beta-Hydroxybutyrate Dehydrogenase (H-010)

Form

Off-white to light gray lyophilized powder.

Activity

≥ 20 U/mg powder.

Unit

One unit is defined as the amount of enzyme, which catalyzes the formation of 1 µmole of NADH per minute at  37˚C under the conditions given in  the assay procedure.

Salicylate Hydroxylase (S-010)

Form

Yellow lyophilized powder.

Activity

≥ 3.5 U/mg powder.

Contaminants

NADH oxidase

≤ 0.7% SH activity.

Unit

The amount of enzyme which catalyzes the conversion of 1 µmole of NADH per minute at 37°C under the conditions given in the assay method.

Arylacylamidase (A-010): Datasheet (MSL200.02 10-2014)
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Beta-Hydroxybutyrate Dehydrogenase (H-010): Datasheet (MSL300.02 10-2014)
Download
Salicylate Hydroxylase (S-010): Datasheet (MSL100.02 10-2014)
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